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anti flot2  (Proteintech)


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    Structured Review

    Proteintech anti flot2
    Anti Flot2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flot2/Flotillin+2+Antibody/pmc12041605-263-96-102
    Average 93 stars, based on 25 article reviews
    anti flot2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway
    Article Snippet: .. The following antibodies for western blotting and immunofluorescence analyses were used: anti-TMEM33 for WB (Abcam, ab242108); anti-TMEM33 for IF (Bethyl, A305-597AT); anti-RTN4 for WB (Proteintech, 10950-1-AP); anti-RTN4 for IF (Santa Cruz, sc-271878); anti-RAB22A (Sigma Aldrich, HPA066920); anti-LC3B for WB (Sigma Aldrich, L7543); anti-LC3B for IF (MBL International, PM036); anti-ATG9A (Abcam, ab108338); anti-ATG16L1 (8089 T), anti-ATG5 (12994) and anti-CD9 (13174) (Cell Signaling Technology); anti-Syntenin-1 (22399-1-AP); anti-ATL3 (16921-1-AP) and anti-Climp63 (16686-1-AP) (Proteintech); anti-CD63 (Beyotime, AF1471); anti-Calnexin (Santa Cruz, sc-46669); anti-TBC1D2B (Santa Cruz, sc-398906); anti-HA-tag (3724S), anti-FLAG-tag (14793S), anti-V5-tag (13202S) (Cell Signaling Technology); anti-GAPDH (Proteintech, 10494-1-AP); anti-β-Tubulin (Bioworld, AP0064); anti-FLOT2 (Cell Signaling Technology, 3436); anti-Alix (Proteintech, 12422-1-AP); anti-Vinculin (Santa Cruz, sc-73614); and secondary antibodies for IF conjugated with Alex Fluor-488 (A-11008), Alex Fluor-594 (A-11005), Alex Fluor-568 (A11036) and Alex Fluor-647 (A21236) were all from Invitrogen. .. The following reagents were used in this study: DSS (Thermo Fisher, A39267); doxycycline (Selleck, S4163); poly-L-lysine (Beyotime, ST509); Hoechst (Invitrogen, 33342); ATP (MCE, HY-B0345A); anti-DYKDDDDK magnetic beads (Thermo Fisher, A36797); DAB (Sigma, D5637); 30% hydrogen peroxide (Sigma, 88597); anti-CD63 magnetic beads (Thermo Fisher, 10606D); anti-streptavidin Sepharose (Cytiva, 17511301); anti-DYKDDDDK agarose (Bimake, B23102); and anti-HA agarose (Bimake, B26201).

    Article Title: The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway.
    Article Snippet: .. The following antibodies for western blotting and immunofluorescence analyses were used: anti-TMEM33 for WB (Abcam, ab242108); anti-TMEM33 for IF (Bethyl, A305-597AT); anti-RTN4 for WB (Proteintech, 10950-1-AP); anti-RTN4 for IF (Santa Cruz, sc-271878); anti-RAB22A (Sigma Aldrich, HPA066920); anti-LC3B for WB (Sigma Aldrich, L7543); anti-LC3B for IF (MBL International, PM036); anti-ATG9A (Abcam, ab108338); anti-ATG16L1 (8089 T), anti-ATG5 (12994) and anti-CD9 (13174) (Cell Signaling Technology); anti-Syntenin-1 (22399-1-AP); anti-ATL3 (16921-1-AP) and antiClimp63 (16686-1-AP) (Proteintech); anti-CD63 (Beyotime, AF1471); antiCalnexin (Santa Cruz, sc-46669); anti-TBC1D2B (Santa Cruz, sc-398906); anti-HA-tag (3724S), anti-FLAG-tag (14793S), anti-V5-tag (13202S) (Cell Signaling Technology); anti-GAPDH (Proteintech, 10494-1-AP); antiβ-Tubulin (Bioworld, AP0064); anti-FLOT2 (Cell Signaling Technology, 3436); anti-Alix (Proteintech, 12422-1-AP); anti-Vinculin (Santa Cruz, sc73614); and secondary antibodies for IF conjugated with Alex Fluor-488 (A11008), Alex Fluor-594 (A-11005), Alex Fluor-568 (A11036) and Alex Fluor647 (A21236) were all from Invitrogen. .. The following reagents were used in this study: DSS (Thermo Fisher, A39267); doxycycline (Selleck, S4163); poly-L-lysine (Beyotime, ST509); Hoechst (Invitrogen, 33342); ATP (MCE, HY-B0345A); anti-DYKDDDDK magnetic beads (Thermo Fisher, A36797); DAB (Sigma, D5637); 30% hydrogen peroxide (Sigma, 88597); anti-CD63 magnetic beads (Thermo Fisher, 10606D); anti-streptavidin Sepharose (Cytiva, 17511301); antiDYKDDDDK agarose (Bimake, B23102); and anti-HA agarose (Bimake, B26201).

    Immunofluorescence:

    Article Title: The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway
    Article Snippet: .. The following antibodies for western blotting and immunofluorescence analyses were used: anti-TMEM33 for WB (Abcam, ab242108); anti-TMEM33 for IF (Bethyl, A305-597AT); anti-RTN4 for WB (Proteintech, 10950-1-AP); anti-RTN4 for IF (Santa Cruz, sc-271878); anti-RAB22A (Sigma Aldrich, HPA066920); anti-LC3B for WB (Sigma Aldrich, L7543); anti-LC3B for IF (MBL International, PM036); anti-ATG9A (Abcam, ab108338); anti-ATG16L1 (8089 T), anti-ATG5 (12994) and anti-CD9 (13174) (Cell Signaling Technology); anti-Syntenin-1 (22399-1-AP); anti-ATL3 (16921-1-AP) and anti-Climp63 (16686-1-AP) (Proteintech); anti-CD63 (Beyotime, AF1471); anti-Calnexin (Santa Cruz, sc-46669); anti-TBC1D2B (Santa Cruz, sc-398906); anti-HA-tag (3724S), anti-FLAG-tag (14793S), anti-V5-tag (13202S) (Cell Signaling Technology); anti-GAPDH (Proteintech, 10494-1-AP); anti-β-Tubulin (Bioworld, AP0064); anti-FLOT2 (Cell Signaling Technology, 3436); anti-Alix (Proteintech, 12422-1-AP); anti-Vinculin (Santa Cruz, sc-73614); and secondary antibodies for IF conjugated with Alex Fluor-488 (A-11008), Alex Fluor-594 (A-11005), Alex Fluor-568 (A11036) and Alex Fluor-647 (A21236) were all from Invitrogen. .. The following reagents were used in this study: DSS (Thermo Fisher, A39267); doxycycline (Selleck, S4163); poly-L-lysine (Beyotime, ST509); Hoechst (Invitrogen, 33342); ATP (MCE, HY-B0345A); anti-DYKDDDDK magnetic beads (Thermo Fisher, A36797); DAB (Sigma, D5637); 30% hydrogen peroxide (Sigma, 88597); anti-CD63 magnetic beads (Thermo Fisher, 10606D); anti-streptavidin Sepharose (Cytiva, 17511301); anti-DYKDDDDK agarose (Bimake, B23102); and anti-HA agarose (Bimake, B26201).

    Article Title: The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway.
    Article Snippet: .. The following antibodies for western blotting and immunofluorescence analyses were used: anti-TMEM33 for WB (Abcam, ab242108); anti-TMEM33 for IF (Bethyl, A305-597AT); anti-RTN4 for WB (Proteintech, 10950-1-AP); anti-RTN4 for IF (Santa Cruz, sc-271878); anti-RAB22A (Sigma Aldrich, HPA066920); anti-LC3B for WB (Sigma Aldrich, L7543); anti-LC3B for IF (MBL International, PM036); anti-ATG9A (Abcam, ab108338); anti-ATG16L1 (8089 T), anti-ATG5 (12994) and anti-CD9 (13174) (Cell Signaling Technology); anti-Syntenin-1 (22399-1-AP); anti-ATL3 (16921-1-AP) and antiClimp63 (16686-1-AP) (Proteintech); anti-CD63 (Beyotime, AF1471); antiCalnexin (Santa Cruz, sc-46669); anti-TBC1D2B (Santa Cruz, sc-398906); anti-HA-tag (3724S), anti-FLAG-tag (14793S), anti-V5-tag (13202S) (Cell Signaling Technology); anti-GAPDH (Proteintech, 10494-1-AP); antiβ-Tubulin (Bioworld, AP0064); anti-FLOT2 (Cell Signaling Technology, 3436); anti-Alix (Proteintech, 12422-1-AP); anti-Vinculin (Santa Cruz, sc73614); and secondary antibodies for IF conjugated with Alex Fluor-488 (A11008), Alex Fluor-594 (A-11005), Alex Fluor-568 (A11036) and Alex Fluor647 (A21236) were all from Invitrogen. .. The following reagents were used in this study: DSS (Thermo Fisher, A39267); doxycycline (Selleck, S4163); poly-L-lysine (Beyotime, ST509); Hoechst (Invitrogen, 33342); ATP (MCE, HY-B0345A); anti-DYKDDDDK magnetic beads (Thermo Fisher, A36797); DAB (Sigma, D5637); 30% hydrogen peroxide (Sigma, 88597); anti-CD63 magnetic beads (Thermo Fisher, 10606D); anti-streptavidin Sepharose (Cytiva, 17511301); antiDYKDDDDK agarose (Bimake, B23102); and anti-HA agarose (Bimake, B26201).



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